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KMID : 1020220210210050545
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Journal of Korean Society of Dental Hygiene
2021 Volume.21 No. 5 p.545 ~ p.553
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Reactive oxygen species-mediated cytotoxicity of indirect restorative cement on periodontal stem cells
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Park So-Yeong
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Abstract
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Objectives: This study aimed to investigate the cytotoxicity of Nexus RMGIC, an indirect restorative cement, on cell survival rate and reactive oxygen species (ROS) production in periodontal stem cells (PDSCs).
Methods: PDSCs were incubated with serially diluted Nexus RMGIC eluates with and without the addition of N-acetyl-cysteine (NAC). Cell survival was determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The ROS generation was determined by measuring the fluorescence intensity for 2′,7′-dichlorofluorescin diacetate.
Results: Nexus RMGIC exposure decreased cell proliferation and cell survival rate in a dose-dependent manner (1:8, 1:4, 1:2, 1:1) in PDSCs. The cytotoxicity of Nexus RMGIC was inhibited by treatment with 10-mM NAC. In addition, the production of ROS was detected by immunofluorescence after PDSCs were exposed to Nexus RMGIC. However, ROS generation was significantly suppressed in the NAC pretreatment compared with the Nexus RMGIC group.
Conclusions: Nexus RMGIC increased the cytotoxicity and ROS generation. ROS was involved in Nexus RMGIC-induced cell toxicity.
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KEYWORD
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Acrylic resin, Cytotoxicity, Dental cement, N-acetyl-L-cysteine, Reactive oxygen species
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